Jung K, Pergande M, Reinholdt C, Schulze B, Scholz D, Strobelt V
J Clin Chem Clin Biochem. 1984 Aug;22(8):523-5. doi: 10.1515/cclm.1984.22.8.523.
The catalytic activities of alanine aminopeptidase, alkaline phosphatase and gamma-glutamyltransferase were determined in the soluble and particulate fractions of urine, after ultracentrifugation. In healthy adults the fractional catalytic activities in the supernatants were 0.53, 0.56 and 0.24, respectively. Nearly the same proportions were found in children. In patients suffering from chronic kidney diseases there was a tendency for the proportion of catalytic activity in the soluble fraction to increase. However, the separation into the multiple forms gave no higher diagnostic reliability than the determination of total catalytic activity of the respective enzymes. The determination of multiple forms has no clinical significance in the detection of rejection episodes in renal transplant recipients.
超速离心后，测定了尿液可溶部分和颗粒部分中丙氨酸氨基肽酶、碱性磷酸酶和γ-谷氨酰转移酶的催化活性。在健康成年人中，上清液中的部分催化活性分别为0.53、0.56和0.24。儿童中也发现了几乎相同的比例。在患有慢性肾病的患者中，可溶部分的催化活性比例有增加的趋势。然而，与测定各酶的总催化活性相比，分离成多种形式并没有更高的诊断可靠性。多种形式的测定在检测肾移植受者的排斥反应中没有临床意义。
