转导蛋白γ亚基的部分cDNA序列。

Partial cDNA sequence of the gamma subunit of transducin.

作者信息

Van Dop C, Medynski D, Sullivan K, Wu A M, Fung B K, Bourne H R

出版信息

Biochem Biophys Res Commun. 1984 Oct 15;124(1):250-5. doi: 10.1016/0006-291x(84)90944-6.

Abstract

A 151 bp cDNA segment that encodes the amino-terminal portion of the gamma subunit of bovine transducin was isolated from a retinal cDNA library constructed in the expression vector lambda gt11. The base sequence of this cDNA confirms the sequence of the first 39 amino-terminal amino acids reported for the native protein (McConnell et al. (1984) Fed. Proc. 43, 1585). Northern blot analysis indicates that the complete mRNA is approximately 650 bases long and that its expression is limited to the retina.

摘要

从构建于表达载体λgt11中的视网膜cDNA文库中分离出一段151bp的cDNA片段，该片段编码牛转导蛋白γ亚基的氨基末端部分。此cDNA的碱基序列证实了已报道的天然蛋白质前39个氨基末端氨基酸的序列（麦康奈尔等人，（1984年）《联邦程序》43卷，第1585页）。Northern印迹分析表明，完整的mRNA约650个碱基长，其表达仅限于视网膜。