Riggall F C, Abrams R M, Kalra P S, Cantor B, Spellacy W N
Department of Gynecology and Obstetrics, University of Florida College of Medicine, Gainesville 32610.
J Reprod Med. 1983 May;28(5):325-9.
The in vivo responsiveness of the ovine fetal and neonatal pituitary was investigated using acute and chronic preparations. Several studies were performed on six fetuses and six lambs from 112 days' gestation through 5 weeks after birth. Ten micrograms of gonadotropin-releasing hormone (GnRH) was injected intravenously into each fetus and lamb, and serial blood samples were collected from the fetus, ewe and lamb. The luteinizing hormone (LH) content of each sample was determined by species-specific radioimmunoassay. Both the fetus and the neonate showed a response to GnRH. A significant elevation of LH was found in all animals by 15 minutes. There was no response in the ewe when GnRH was injected into its fetus. Great variability in response was noted among various age groups and between animals, with the maximum individual LH response reaching 33.7 ng/ml. The maximum LH response of the neonate during the first week of life was significantly depressed (p < 0.05) as compared to that of the fetus and the older neonate.
利用急性和慢性制剂研究了绵羊胎儿和新生羊垂体的体内反应性。对6只胎儿和6只羔羊进行了多项研究，涵盖从妊娠112天到出生后5周的阶段。向每只胎儿和羔羊静脉注射10微克促性腺激素释放激素（GnRH），并从胎儿、母羊和羔羊采集系列血样。通过种属特异性放射免疫测定法测定每个样本中的促黄体生成素（LH）含量。胎儿和新生儿均对GnRH有反应。15分钟时在所有动物中均发现LH显著升高。向母羊的胎儿注射GnRH时，母羊无反应。在不同年龄组之间以及动物之间观察到反应存在很大差异，个体LH的最大反应达到33.7 ng/ml。与胎儿和较大的新生儿相比，新生儿出生后第一周的最大LH反应显著降低（p<0.05）。
