Bentwood B J, Henson P M
J Immunol. 1980 Feb;124(2):855-62.
The degranulation response of human neutrophils to the calcium ionophore A23187, serum opsonized zymosan (ZC), aggregated gamma-globulin (A gamma G), C5a, formyl-methionyl-leucyl-phenylalanine (FMLP), and PMA has been studied as a reaction time course in order to compare the release kinetics of the separate granule types. Cell suspensions were treated with submaximal doses of stimuli for various time intervals, and the isolated supernatants were assayed for granule constituents. Lactoferrin (LF), a unique specific (secondary) granule protein, was measured by radioimmune assay, and the azurophil (primary) granule components, myeloperoxidase (MPO) and beta-glucuronidase (beta-glu), by enzymatic activity. A sequential pattern of first LF release followed by MPO and beta-glu was demonstrated with each of the stimuli examined, with or without cytochalasin B pretreatment. These kinetic studies demonstrate that the extracellular release of the specific and azurophil granules occur sequentially in human neutrophils with both soluble and particulate stimuli. These findings support the concept that the two granule types are subject to separate controlling factors.
为了比较不同颗粒类型的释放动力学,研究了人类中性粒细胞对钙离子载体A23187、血清调理酵母聚糖(ZC)、聚合γ球蛋白(AγG)、C5a、甲酰甲硫氨酰亮氨酰苯丙氨酸(FMLP)和佛波酯(PMA)的脱颗粒反应的时间进程。用亚最大剂量的刺激物处理细胞悬液不同时间间隔,对分离的上清液进行颗粒成分分析。乳铁蛋白(LF)是一种独特的特异性(次级)颗粒蛋白,通过放射免疫测定法测量,而嗜天青(初级)颗粒成分髓过氧化物酶(MPO)和β-葡萄糖醛酸酶(β-葡)则通过酶活性测定。在每种所检测的刺激物处理下,无论有无细胞松弛素B预处理,都显示出先释放LF,随后释放MPO和β-葡的顺序模式。这些动力学研究表明,在人类中性粒细胞中,特异性颗粒和嗜天青颗粒的细胞外释放,在可溶性和颗粒性刺激下都是按顺序发生的。这些发现支持了这两种颗粒类型受不同控制因素影响的概念。