Palmer R M, Salmon J A
Immunology. 1983 Sep;50(1):65-73.
The release of leukotriene B4 (LTB4) from human neutrophils and its relationship to degranulation induced by the divalent cation ionophore A23187, serum-treated zymosan (STZ), N-formyl-methionyl-leucyl-phenylalanine (FMLP) and arachidonic acid (AA) have been studied. Greatest release of LTB4, measured by specific radioimmunoassay, occurred in response to A23187 (5-10 ng/10(6) cells); lower concentrations were obtained after incubation with STZ (0.2-0.8 ng/10(6) cells) and AA (0.3-2.6 ng/10(6) cells) and low (0.02 ng/10(6) cells) or not detectable amounts from cells incubated with FMLP. Release of LTB4 induced by STZ, FMLP and submaximal concentrations of A23187 was potentiated by simultaneous addition of AA. Lower amounts (0.06-0.3 ng/10(6) cells) of thromboxane B2 (TXB2) were also released by these stimuli, however this release of TXB2 was not potentiated by exogenous AA. The secretion of beta-glucuronidase induced by A23187, STZ and FMLP was not quantitatively related to release of LTB4 or TXB2 and was not potentiated by exogenous AA. Furthermore, FMLP induced degranulation was cytochalasin B (Cyt B)-dependent, whereas LTB4 release in response to this stimulus was only marginally increased by pretreatment of the cells with Cyt B. These data indicate that LTB4 does not mediate degranulation induced by these stimuli.
对人中性粒细胞中白三烯B4（LTB4）的释放及其与二价阳离子载体A23187、血清处理的酵母聚糖（STZ）、N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）和花生四烯酸（AA）诱导的脱颗粒之间的关系进行了研究。通过特异性放射免疫测定法测得，LTB4释放量最大的情况出现在对A23187（5 - 10 ng/10⁶细胞）的反应中；与STZ（0.2 - 0.8 ng/10⁶细胞）和AA（0.3 - 2.6 ng/10⁶细胞）孵育后得到的浓度较低，而与FMLP孵育的细胞中释放量低（0.02 ng/10⁶细胞）或检测不到。同时添加AA可增强STZ、FMLP和亚最大浓度A23187诱导的LTB4释放。这些刺激也会释放较少量（0.06 - 0.3 ng/10⁶细胞）的血栓素B2（TXB2），然而外源性AA并不能增强TXB2的这种释放。A23187、STZ和FMLP诱导的β-葡萄糖醛酸酶分泌与LTB4或TXB2的释放没有定量关系，且外源性AA也不能增强其分泌。此外，FMLP诱导的脱颗粒依赖于细胞松弛素B（Cyt B），而用Cyt B预处理细胞仅略微增加了对该刺激的LTB4释放。这些数据表明LTB4并不介导这些刺激诱导的脱颗粒。
