Mishler J M, Ricketts C R, Parkhouse E J
J Clin Pathol. 1980 Feb;33(2):155-9. doi: 10.1136/jcp.33.2.155.
Intravascular persistence concomitant with changes occurring in the circulating molecular size distribution were determined in five healthy normovolaemic men dosed with 7 ml/kg of a 6% (w/v) solution of a species of hydroxyethyl starch (HES 450/0.70) possessing a M(w)- of 450 000 daltons combined with a molar hydroxyethyl group substitution (MS) of 0.70 (70 hydroxyethyl groups/100 glucose residues). The concentration of HES 450/0.70 in serum fell to 24% of its peak value (measured 2 minutes post injection) one week after the infusion. By 17 weeks after injection, < 1.0% remained in the intravascular space. The HES 450/0.70 material recovered from the bloodstream 2 minutes after injection was shown by gel filtration on a column of Sepharose CL-4B to be less polydisperse than the injected solution. The K(av) calculated for the peak of material eluted after one week showed a definite shift of molecular size toward that of a lower molecular weight composition. However, at four weeks the value of K(av) indicated a shift toward the high molecular weight region of the injected solution, and by seven weeks this movement was quite pronounced. These data clearly indicate the complex nature of the removal of HES 450/0.70 from the intravascular space of man and appear to substantiate previous clinical studies reporting that the MS plays the major role influencing the rate of elimination of this material from the bloodstream.
在5名健康的血容量正常男性中，静脉注射7 ml/kg的6%（w/v）某一羟乙基淀粉（HES 450/0.70）溶液，其Mw为450000道尔顿，羟乙基基团摩尔取代度（MS）为0.70（70个羟乙基基团/100个葡萄糖残基），测定血管内的持续存在情况以及循环分子大小分布的变化。输注后一周，血清中HES 450/0.70的浓度降至其峰值（注射后2分钟测得）的24%。注射后17周，血管内剩余量<1.0%。注射后2分钟从血流中回收的HES 450/0.70物质，经Sepharose CL-4B柱凝胶过滤显示，其多分散性低于注射溶液。计算得出一周后洗脱物质峰值的K(av)，表明分子大小明显向较低分子量组成方向偏移。然而，四周时K(av)值表明向注射溶液的高分子量区域偏移，到七周时这种偏移相当明显。这些数据清楚地表明了从人体血管内清除HES 450/0.70的复杂性质，似乎证实了先前的临床研究报告，即MS在影响该物质从血流中消除速率方面起主要作用。
