Schroeder L L, Pappas P W
J Parasitol. 1980 Feb;66(1):49-52.
Significant amounts of radioactivity were associated with Hymenolepis diminuta following incubation in 3H-trypsin. Autoradiography of worms incubated in 3H-trypsin for 30 min demonstrated that all radioactivity was associated with the worm's surface (tegument). The amount of 3H-trypsin adsorbed by the worms was not sufficient to account for the inactivation of this enzyme in the presence of intact worms. Unlabeled trypsin and poly-L-glutamate (but not poly-L-lysine) inhibited adsorption of 3H-trypsin, but were without effect on trypsin inactivation by H. diminuta. Therefore, trypsin was adsorbed by intact H. diminuta, but the process of adsorption apparently did not play any role in inactivation of the enzyme.
在3H-胰蛋白酶中孵育后，微小膜壳绦虫与大量放射性相关。在3H-胰蛋白酶中孵育30分钟的虫体放射自显影显示，所有放射性都与虫体表面（皮层）相关。在完整虫体存在的情况下，虫体吸附的3H-胰蛋白酶量不足以解释该酶的失活。未标记的胰蛋白酶和聚-L-谷氨酸（但不是聚-L-赖氨酸）抑制3H-胰蛋白酶的吸附，但对微小膜壳绦虫使胰蛋白酶失活没有影响。因此，胰蛋白酶被完整的微小膜壳绦虫吸附，但吸附过程显然在酶的失活中没有起到任何作用。
