Ribeiro-Da-Silva A, Coimbra A
Brain Res. 1980 Apr 28;188(2):449-64. doi: 10.1016/0006-8993(80)90044-x.
[3H]GABA or [3H]glycine were injected into the subarachnoidal space of adult rats at C4-C5 level. After 10-60 min, the animals were perfused with 4% paraformaldehyde-0.5% glutaraldehyde and thick sections of the cervical spinal cord were postosmicated and Epon embedded. Light microscope autoradiographs of transverse cord sections showed numerous silver grains over the dorsal column and laminae I-III, higher grain densities occurring over lamina I for GABA and lamina III for glycine. In [3H]GABA-injected animals nerve cell bodies in lamina I or at the transition to lamina II appeared strongly labeled in light and electron microscope autoradiographs. These cells were smaller and less rich in RER than marginal cells and poor in axosomatic synaptic contacts. High grain densities appeared over axon terminals synapsing with dendrites in laminae I-II and over the light peripheral axon endings of synaptic glomeruli of laminae II-III. After [3H]glycine treatment, a number of nerve cell bodies were labeled in lamina III. It is suggested that two types of inhibitory interneurons occur in the rat gelatinosa, one GABAergic with cell body in lamina I, and another glycinergic in lamina III.
将[³H]γ-氨基丁酸（GABA）或[³H]甘氨酸注入成年大鼠C4 - C5水平的蛛网膜下腔。10 - 60分钟后，用4%多聚甲醛 - 0.5%戊二醛灌注动物，将颈脊髓的厚切片进行后固定并包埋在环氧树脂中。脊髓横切片的光学显微镜放射自显影片显示，在背柱和板层I - III上有大量银颗粒，GABA在板层I上的颗粒密度较高，甘氨酸在板层III上的颗粒密度较高。在注射[³H]GABA的动物中，板层I或向板层II过渡处的神经细胞体在光学和电子显微镜放射自显影片中显示出强烈标记。这些细胞比边缘细胞小，粗面内质网较少，轴体突触联系也较少。在板层I - II中与树突形成突触的轴突终末以及板层II - III突触小球的轻度外周轴突终末上出现高颗粒密度。在[³H]甘氨酸处理后，板层III中有许多神经细胞体被标记。提示大鼠胶状质中存在两种类型的抑制性中间神经元，一种是GABA能的，细胞体在板层I；另一种是甘氨酸能的，在板层III。
