人胰腺分泌型胰蛋白酶抑制剂的放射免疫测定：正常受试者和胰腺疾病患者血清胰腺分泌型胰蛋白酶抑制剂的测定

Radioimmunoassay for human pancreatic secretory trypsin inhibitor: measurement of serum pancreatic secretory trypsin inhibitor in normal subjects and subjects with pancreatic diseases.

作者信息

Kitahara T, Takatsuka Y, Fujimoto K I, Tanaka S, Ogawa M, Kosaki G

出版信息

Clin Chim Acta. 1980 Apr 25;103(2):135-43. doi: 10.1016/0009-8981(80)90205-3.

Abstract

A reliable radioimmunoassay (RIA) for human pancreatic secretory trypsin inhibitor (PSTI) has been developed. The method is highly sensitive (0.4 ng/ml), reproducible and specific. A good parallel relationship was observed between the standard curve and dilution curves for serum and urine. The PSTI bound to trypsin-alpha 2-macroglobulin complexes was found not to be immunoreactive, whereas a part of the psti-trypsin complex was immuno-reactive. In healthy individuals, serum PSTI level ranged from 5.4 ng/ml to 16.0 ng/ml, the average being 11.3 ng/ml (S.D. +/- 2.7). Elevated values were observed in patients with acute pancreatitis (highest value 3200 ng/ml), and in some patients with chronic relapsing pancreatitis.

摘要

已开发出一种用于检测人胰腺分泌型胰蛋白酶抑制剂（PSTI）的可靠放射免疫分析法（RIA）。该方法高度灵敏（0.4 ng/ml）、可重复且具有特异性。在标准曲线与血清和尿液的稀释曲线之间观察到良好的平行关系。发现与胰蛋白酶-α2-巨球蛋白复合物结合的PSTI无免疫反应性，而部分PSTI-胰蛋白酶复合物具有免疫反应性。在健康个体中，血清PSTI水平范围为5.4 ng/ml至16.0 ng/ml，平均为11.3 ng/ml（标准差±2.7）。在急性胰腺炎患者中观察到值升高（最高值3200 ng/ml），并且在一些慢性复发性胰腺炎患者中也有升高。