Kemali M, Guglielmotti V, Gioffré D
Exp Brain Res. 1980 Feb;38(3):341-7. doi: 10.1007/BF00236654.
A study of the habenular nuclei connections by means of horseradish peroxidase (HRP) has never been carried out in amphibia. In the present paper we have investigated the afferent projections of the left and right habenular nuclei of the frog Rana esculenta using this technique. Cells, labelled by HRP, were either in a Golgi-like pattern or in a granular pattern. It appears that the habenular nuclei on the two sides of the epithalamus do not show different connections even though they are morphologically asymmetric. In fact, each habenula is connected bilaterally with the septal area and the bed nucleus of the hippocampal commissure, and ipsilaterally with the hypothalamic areas, the entopeduncular nucleus, the periventricular gray of the third ventricle and the interpeduncular nucleus. However, the habenular commissure has typical commissural fibres which apparently do not involve the medial portion of the left habenula. The habenular connections in the frog are generally similar to those reported in the literature for mammals. In addition, our results show the possibility that HRP is transported both retrograde and anterograde.
尚未在两栖动物中利用辣根过氧化物酶（HRP）对缰核连接进行过研究。在本文中，我们运用该技术研究了食用蛙（Rana esculenta）左右缰核的传入投射。被HRP标记的细胞呈高尔基样模式或颗粒状模式。尽管上丘脑两侧的缰核在形态上不对称，但似乎它们并未表现出不同的连接。事实上，每个缰核都与隔区和海马连合的终纹床核双侧相连，并与下丘脑区域、内脚核、第三脑室室周灰质和脚间核同侧相连。然而，缰连合具有典型的连合纤维，显然不涉及左侧缰核的内侧部分。蛙的缰核连接总体上与文献中报道的哺乳动物的缰核连接相似。此外，我们的结果表明HRP可能进行逆行和顺行运输。
