Malis F, Kasafírek E, Fric P, Josífko M
Acta Univ Carol Med Monogr. 1977(79 Pt 3):131-8.
The newly synthesized chromogenic substrate Nalpha-tosyl-L-arginine-p-nitroanilide (TAPA) was used to determine the trypsin activity (as the concentration and output in 20-min samples) of the duodenal contents before and after hormonal stimulation with cholecystokinin-pancreozymin (CPZ) and secretin (S). The results were compared with those obtained by means of Nalpha-benzoyl-D,L-arginine-p-nitroanilide (BAPA) and the Nalpha-tosyl-L-arginine methylester (TAME). The synthetic substrates were compared in 17 subjects with normal and in 8 subjects with pathological CPZ-S test values. A high degree of correlation was found in every case between the values obtained by means of the given substrates and it actually proved possible to determine the conversion coefficients for the trypsin concentration (BAPA = 1.306 TAPA, TAME = 0.509 TAPA) and for trypsin output (BAPA = 1.306 TAPA, TAME = 0.535 TAPA). The results demonstrate the usefulness of our new chromogenic substrate TAPA, which is more readily available and cheaper than BAPA and TAME for determining trypsin activity in biological material.
新合成的显色底物Nα-对甲苯磺酰-L-精氨酸对硝基苯胺（TAPA）用于测定在胆囊收缩素-促胰酶素（CPZ）和促胰液素（S）激素刺激前后十二指肠内容物的胰蛋白酶活性（以20分钟样本中的浓度和产量表示）。将结果与通过Nα-苯甲酰-D，L-精氨酸对硝基苯胺（BAPA）和Nα-对甲苯磺酰-L-精氨酸甲酯（TAME）获得的结果进行比较。在17名CPZ-S测试值正常的受试者和8名CPZ-S测试值病理性的受试者中对合成底物进行了比较。在每种情况下，通过给定底物获得的值之间都发现了高度相关性，实际上证明可以确定胰蛋白酶浓度（BAPA = 1.306 TAPA，TAME = 0.509 TAPA）和胰蛋白酶产量（BAPA = 1.306 TAPA，TAME = 0.535 TAPA）的转换系数。结果证明了我们新的显色底物TAPA的实用性，它比BAPA和TAME更易于获得且更便宜，可用于测定生物材料中的胰蛋白酶活性。
