Mishler J M, Ricketts C R, Parkhouse E J
Int J Clin Pharmacol Ther Toxicol. 1980 Mar;18(3):120-1.
Fragments of low molecular weight-hydroxyethylated amylopectin (LMW-HES) contained in the urine voided by a single normal man, 6 to 12 hours after receiving a dose of 56 g of this material, were either separated straightforwardly by gel filtration on a column of Sephadex G-200 or incubated for 18 hours at 37 degrees C in the presence of alpha-amylase contained in human saliva, and then subjected to filtration on the same column. Comparison of the two elution profiles revealed that LMW-HES material remaining in the bloodstream for 6-hours or more, had not been degraded by serum alpha-amylase to the maximum possible extent, since incubation with alpha-amylase in-vitro caused further catabolism. These data suggest that LMW-HES polymer fragments are excreted in the urine as soon as they obtain a molecular weight below the threshold of glomerular filtration.
一名正常男性在摄入56克低分子量羟乙基化支链淀粉（LMW-HES）后6至12小时排出的尿液中所含的LMW-HES片段，要么通过在Sephadex G-200柱上进行凝胶过滤直接分离，要么在含有人类唾液中α-淀粉酶的情况下于37℃孵育18小时，然后在同一柱上进行过滤。两种洗脱图谱的比较显示，在血液中留存6小时或更长时间的LMW-HES物质，并未被血清α-淀粉酶最大限度地降解，因为体外与α-淀粉酶孵育会导致进一步的分解代谢。这些数据表明，LMW-HES聚合物片段一旦分子量低于肾小球滤过阈值，就会随尿液排出。
