Ramshaw I A, Woodsworth M, Wright K, McKenzie I F
J Immunol. 1980 Jul;125(1):197-201.
These studies describe the conditions under which antibody-forming cells and TDTH cells are selectively induced in vitro. TDTH cells are preferentially stimulated when high doses of antigen are included in the culture. Antibody-forming cells, on the other hand, are optimally stimulated with a 100 to 1000-fold less concentration of antigen. The conditions that optimally stimulate TDTH cells also induce a population of suppressor T cells that inhibit the antibody response. However, although their inductive requirements are similar, the suppressor T cells of antibody formation are a distinct subpopulation of cells from the TDTH cells. Whereas the suppressor T cells are LY-1-, 2+, 4-, 6+, and Ia+; the TDTH cells are Ly-1+, 2+/-, 4-, 6+, and Ia-. Furthermore, the DTH cells are sensitive to high doses of irradiation, whereas the suppressor cells are resistant. Based on the Ly phenotype and the kinetics of suppression, the suppressor T cells are not the "feedback suppressors" that have been identified in other systems. The system described in this paper provides a means whereby the cells that regulate humoral and CMI can be studied in vitro.
这些研究描述了在体外选择性诱导抗体形成细胞和迟发型超敏反应性T细胞（TDTH细胞）的条件。当培养物中含有高剂量抗原时，TDTH细胞受到优先刺激。另一方面，抗体形成细胞在抗原浓度低100至1000倍时受到最佳刺激。最佳刺激TDTH细胞的条件也会诱导一群抑制抗体反应的抑制性T细胞。然而，尽管它们的诱导需求相似，但抑制抗体形成的抑制性T细胞是与TDTH细胞不同的细胞亚群。抑制性T细胞为LY-1-、2+、4-、6+和Ia+；而TDTH细胞为Ly-1+、2+/-、4-、6+和Ia-。此外，迟发型超敏反应细胞对高剂量辐射敏感，而抑制性细胞具有抗性。基于Ly表型和抑制动力学，抑制性T细胞不是在其他系统中已鉴定出的“反馈抑制因子”。本文所述系统提供了一种在体外研究调节体液免疫和细胞介导免疫的细胞的方法。
