Sherman L A
J Exp Med. 1980 Jun 1;151(6):1386-97. doi: 10.1084/jem.151.6.1386.
The B10.D2 cytolytic T lymphocyte (CTL) receptor repertoire specific for the H-2Kb alloantigen has been studied by determining the reactivity patterns of monoclonal CTL against a panel of seven different H-2Kb mutants. The repertoire is extremely diverse and contains a minimum of approximately 50 different specificities against unique antigenic determinants on the H-2Kb molecule. Each specificity appears at a maximum frequency of 1 in 18,000 CTL precursors. These studies have also served to dissect the antigenic composition of the H-2Kb molecule. Very few CTL clonotypes share recognition of all the mutants, thereby indicating the lack of conservation of a b-type antigenic region. In addition, the degree to which each mutant shares antigenic determinants with the standard H-2Kb molecule has been determined.
通过测定单克隆细胞毒性T淋巴细胞(CTL)对一组七种不同的H-2Kb突变体的反应模式,研究了针对H-2Kb同种异体抗原的B10.D2细胞毒性T淋巴细胞(CTL)受体库。该受体库极其多样,至少包含约50种针对H-2Kb分子上独特抗原决定簇的不同特异性。每种特异性在CTL前体细胞中的出现频率最高为1/18000。这些研究还有助于剖析H-2Kb分子的抗原组成。很少有CTL克隆型对所有突变体都具有共同的识别能力,从而表明缺乏b型抗原区域的保守性。此外,还确定了每个突变体与标准H-2Kb分子共享抗原决定簇的程度。