Lin C C, Jorgenson K F, van de Sande J H
Chromosoma. 1980;79(3):271-86. doi: 10.1007/BF00327319.
Metaphase chromosomes stained with acridine orange exhibit uniform yellow-green fluorescence. Chromosome preparations treated with the non-fluorescent A-T specific antibiotic distamycinA prior to acridine orange staining exhibit longitudinal fluorescent banding patterns similar to those produced by a number of fluorescent R-band techniques. Similarly, chromosome preparations treated with the non-fluorescent G-C specific antibiotic actinomycin D followed by acridine orange staining exhibit "Hoechst-type" banding patterns. Interactions of various ligand-DNA combinations in solution indicate that the base pair specific antibiotics induce banding patterns by selectively altering acridine orange binding sites in chromosomal regions rich in the particular base pair for which the antibiotic exhibits specificity.
用吖啶橙染色的中期染色体呈现均匀的黄绿色荧光。在用吖啶橙染色之前，先用非荧光的A-T特异性抗生素偏端霉素A处理染色体标本，会呈现出与多种荧光R带技术产生的纵向荧光带型相似的带型。同样，先用非荧光的G-C特异性抗生素放线菌素D处理染色体标本，然后进行吖啶橙染色，会呈现出“Hoechst型”带型。溶液中各种配体与DNA组合的相互作用表明，碱基对特异性抗生素通过选择性改变染色体区域中富含该抗生素所具有特异性的特定碱基对的吖啶橙结合位点来诱导带型。
