Schmid M, Poppen A, Engel W
Cytogenet Cell Genet. 1981;30(4):211-21. doi: 10.1159/000131612.
Lymphocyte cultures from the gorilla, chimpanzee, and orangutan were treated with the oligopeptide antibiotic distamycin A. This AT-specific DNA-ligand induces a distinct undercondensation in the quinacrine-bright heterochromatin of the gorilla and chimpanzee. This is also the case in human lymphocyte cultures. Distamycin A further causes an undercondensation in the nonheterochromatic bands 17q21 of the gorilla and 16q22 of man. No visible distamycin A-sensitive chromosome regions are determined in the orangutan. The in vitro treatment with distamycin A preserves the somatic pairings between the quinacrine-bright heterochromatic regions existing in the interphase nucleus until the succeeding metaphase stage. The phylogenetic origin of the quinacrine-bright and distamycin A-sensitive heterochromatin in the ancestor of man, the gorilla, and the chimpanzee is discussed.
对大猩猩、黑猩猩和猩猩的淋巴细胞培养物用寡肽抗生素偏端霉素A进行处理。这种特异性结合AT的DNA配体在大猩猩和黑猩猩的喹吖因亮型异染色质中诱导出明显的凝缩不足。人类淋巴细胞培养物中也出现这种情况。偏端霉素A还会导致大猩猩17q21和人类16q22的非异染色质带出现凝缩不足。在猩猩中未确定可见的对偏端霉素A敏感的染色体区域。用偏端霉素A进行体外处理可使间期核中存在的喹吖因亮型异染色质区域之间的体细胞配对保留到随后的中期阶段。文中讨论了人类、大猩猩和黑猩猩共同祖先中喹吖因亮型且对偏端霉素A敏感的异染色质的系统发育起源。
