Marcollet M, Morin J, Lecher P
Stain Technol. 1980 Jan;55(1):35-8. doi: 10.3109/10520298009067893.
To study the binding of an antiadenosine serum to human chromosome DNA, two types of chromogenic reagents were compared. The procedure is as follows: lymphocytes with metaphase chromosomes were spread on slides; some slides were irradiated by UV; all slides were then incubated with antiadenosine rabbit serum and then with antirabbit sheep serum labelled with peroxidase; the latter was revealed in the classical manner by 3,3'-diaminobenzidine (DAB), or, alternatively, by p-phenylenediamine plus pyrocatechol (PPD-PC). The present study shows that the results obtained with PPD-PC were equivalent, if not superior, to those obtained with DAB. In the case of weaker reactions, results with PPD-PC were superior. Furthermore, this reagent has the major advantage of being noncarcinogenic.
为研究抗腺苷血清与人染色体DNA的结合情况，对两种显色试剂进行了比较。实验步骤如下：将带有中期染色体的淋巴细胞铺在载玻片上；部分载玻片用紫外线照射；然后所有载玻片先用抗腺苷兔血清孵育，再用标记有过氧化物酶的抗兔羊血清孵育；后者通过3,3'-二氨基联苯胺（DAB）以经典方式显色，或者用对苯二胺加邻苯二酚（PPD-PC）显色。本研究表明，PPD-PC获得的结果即使不比DAB获得的结果更好，至少也是相当的。在反应较弱的情况下，PPD-PC的结果更优。此外，该试剂的主要优点是无致癌性。
