Mel'nikova E V, Karnaukhov V N
Tsitologiia. 1980 Apr;22(4):434-40.
Using the microspectrofluorimetric technique, the temperature dependence of the synthetic activity of the nucleus and of some cytoplasmic areas in the giant identified neurons of Lymnaea stagnalis was investigated after Acridine Orange staining. The elevation of the environmental temperature from 4 degrees to 20 degrees C resulted in the increased synthetic activity of the nucleus and cytoplasm of giant neurons. In these conditions, the highest synthetic activity was registered in the apical area of the neuron cytoplasm, providing presumably, the cell neurosecretory activity. The synthetic activity of the axon perinuclear area, producing compounds for the axonal transport, increases at the elevated temperatures to a lesser degree, because it has a relatively high level as well at low temperatures.
运用显微分光荧光测定技术,在吖啶橙染色后,研究了椎实螺大型特定神经元中细胞核及部分细胞质区域合成活性的温度依赖性。环境温度从4摄氏度升高至20摄氏度,导致大型神经元的细胞核及细胞质合成活性增强。在这些条件下,神经元细胞质顶端区域的合成活性最高,这大概为细胞的神经分泌活动提供了支持。产生用于轴突运输化合物的轴突核周区域的合成活性,在温度升高时增加幅度较小,因为它在低温时也具有相对较高的水平。