聚丙烯酰胺凝胶电泳分离后用于蛋白质中巯基的特异性染色剂。
A specific stain for sulfhydryl groups in proteins after separation by polyacrylamide gel electrophoresis.
作者信息
Telser A, Rovin B
出版信息
Biochim Biophys Acta. 1980 Aug 21;624(2):363-71. doi: 10.1016/0005-2795(80)90077-x.
PMID:6158337
Abstract
A new method is described for specifically staining protein sulfhydryl groups after the proteins have been separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in slab gels. The stain will detect as little as 0.25 microgram of lysozyme and 1 microgram of most other proteins; the range of sensitivity for a specific protein depending on its sulfhydryl content. Proteins with no cysteine residues (type I collagen) and glycoproteins do not cause spurious staining.
摘要
本文描述了一种新方法，用于在蛋白质通过十二烷基硫酸钠-聚丙烯酰胺平板凝胶电泳分离后，特异性地对蛋白质巯基进行染色。该染色剂能检测到低至0.25微克的溶菌酶和1微克的大多数其他蛋白质；特定蛋白质的灵敏度范围取决于其巯基含量。不含半胱氨酸残基的蛋白质（I型胶原蛋白）和糖蛋白不会引起假染色。
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