Shimizu K, Wilson J B, Huisman T H
Hemoglobin. 1980;4(3-4):487-96. doi: 10.3109/03630268008996229.
A newly developed HPLC procedure (1) was used to study the percentages of G gamma and A gamma chains in the Hb F from newborn and patients with sickle cell anemia. The method gives data comparable to those obtained with a more laborious chemical procedure. However, the presence of the A gamma T chain, i.e., the A gamma chain with an Ile replaced by Thr substitution at position 75, interferes with the determination of the G gamma to A gamma ratio because the A gamma T and G gamma chains have the same chromatographic mobility.
采用一种新开发的高效液相色谱法（1）来研究新生儿和镰状细胞贫血患者的Hb F中Gγ和Aγ链的百分比。该方法给出的数据与通过更繁琐的化学方法获得的数据相当。然而，AγT链的存在，即在第75位异亮氨酸被苏氨酸取代的Aγ链，会干扰Gγ与Aγ比值的测定，因为AγT链和Gγ链具有相同的色谱迁移率。
