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[鸽类红细胞细胞核28S前体mRNA和细胞质RNA中的珠蛋白编码序列]

[Globin-coding sequences in the nuclear 28S pre-mRNA and in the cytoplasmic RNA of pigeon erythroid cells].

作者信息

Gazarian K G, Dubovaia V I, Neznanov N S, Tarantul V Z, Skobeleva N A

出版信息

Mol Biol (Mosk). 1980 Jul-Aug;14(4):766-72.

PMID:6158673
Abstract

The steady-state content of globin-coding sequences in nuclear and cytoplasmic RNA of pigeon erythroid cells was estimated by hybridization in the excess of nuclear 28S RNA and cytoplasmic poly(A) + RNA with [3H]DNA, synthesized on globin mRNA. Sequences of 9S globin mRNA are found in 0.06% of molecules of non-ribosomal 28S nuclear RNA (pre-mRNA) of erythroblasts and in 0.5% of molecules of non-ribosomal 28S nuclear RNA of reticulocytes. The content of globin mRNA in erythroblast cytoplasm is, respectively lower than in that of reticulocytes.

摘要

通过用在珠蛋白信使核糖核酸上合成的[3H]DNA与过量的核28S核糖核酸和细胞质多聚腺苷酸(poly(A))+核糖核酸杂交,估算了鸽红细胞核和细胞质核糖核酸中珠蛋白编码序列的稳态含量。在成红细胞非核糖体28S核核糖核酸(前体信使核糖核酸)的0.06%分子中以及网织红细胞非核糖体28S核核糖核酸的0.5%分子中发现了9S珠蛋白信使核糖核酸序列。成红细胞细胞质中珠蛋白信使核糖核酸的含量分别低于网织红细胞中的含量。

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