Petralia S, Soprano K J, Pochron S, Darzynkiewicz Z, Traganos F, Baserga R
Cancer Res. 1980 Sep;40(9):3177-80.
The amount of RNA per cell has been measured by flow microfluorometry in cultured cells stimulated by serum or infected with DNA oncogenic viruses (SV40, polyoma virus, and adenovirus). While all four agents stimulate, although to a different extent, cellular DNA synthesis in quiescent cells, the accumulation of cellular RNA varies. Serum, SV40, and polyoma virus cause a marked increase in total cellular RNA that is already apparent in G1 cells before entry into S. On the other hand, adenovirus 2, while capable of stimulating cellular DNA synthesis, fails to detectably increase the amount of RNA per cell. These results suggest that adenovirus 2 may act on quiescent cells through mechanisms different from those of serum, SV40, or polyoma virus.
已通过流式细胞荧光测定法，对受血清刺激或感染DNA致癌病毒（SV40、多瘤病毒和腺病毒）的培养细胞中的RNA含量进行了测量。虽然所有这四种因子，尽管程度不同，但均能刺激静止细胞中的细胞DNA合成，然而细胞RNA的积累情况却有所不同。血清、SV40和多瘤病毒会导致细胞总RNA显著增加，这种增加在进入S期之前的G1期细胞中就已明显可见。另一方面，腺病毒2虽然能够刺激细胞DNA合成，但却未能显著增加每个细胞的RNA含量。这些结果表明，腺病毒2可能通过与血清、SV40或多瘤病毒不同的机制作用于静止细胞。
