Colletta G, Di Fiore P P, Ferrentino M, Pietropaolo C, Turco M C, Vecchio G
Cancer Res. 1980 Sep;40(9):3369-73.
Tumor-promoting agents are known to inhibit the specific differentiation processes of several animal cell systems in vitro, including the Friend leukemia cell system. We have examined the effect of 12-O tetradecanoylphorbol-13-acetate (TPA) on the latter system and have investigated its action on Friend virus expression. At a concentration of 16.7 nM, TPA inhibits the dimethyl sulfoxide-induced Friend cell terminal differentiation and, at the same time, enhances the expression of the Friend virus genome, as demonstrated by a 2-fold increase in the amount of reverse transcriptase-containing particles released into the culture fluid and in the levels of virus-specific intracytoplasmic RNA. The greatest effect of TPA is evident after 24 hr of treatment. At this time, TPA exerts also its strongest effect upon the induction of the plasminogen activator. Our results indicate that two specific effects of TPA, i.e., block of differentiation and induction of plasminogen activator, correlate well in the Friend cell system with an extracellular and intracellular increase in virus expression.
已知肿瘤促进剂在体外可抑制多种动物细胞系统的特定分化过程，包括弗瑞德白血病细胞系统。我们研究了12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）对后一系统的影响，并探讨了其对弗瑞德病毒表达的作用。在浓度为16.7 nM时，TPA抑制二甲基亚砜诱导的弗瑞德细胞终末分化，同时增强弗瑞德病毒基因组的表达，这表现为释放到培养液中的含逆转录酶颗粒数量增加2倍以及病毒特异性胞浆内RNA水平升高。TPA的最大作用在处理24小时后明显。此时，TPA对纤溶酶原激活物的诱导也发挥最强作用。我们的结果表明，TPA的两种特定作用即分化阻断和纤溶酶原激活物诱导在弗瑞德细胞系统中与病毒表达在细胞外和细胞内增加密切相关
