血红蛋白S被其他血红蛋白溶解。
Solubilization of hemoglobin S by other hemoglobins.
作者信息
Benesch R E, Edalji R, Benesch R, Kwong S
出版信息
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5130-4. doi: 10.1073/pnas.77.9.5130.
Abstract
The polymerization of mixtures of Hb S with hemoglobins A, A2, and F has been investigated by analysis of the proportions of S and non-S hemoglobin both in the supernate and in the pellet after centrifugation. In all cases the non-S hemoglobin was incorporated into the polymer even in the absence of hybrids in the order A > A2 > F. The solubility of Hb S is substantially increased by the other hemoglobins, especially by Hb F, which would account for its antisickling effect. It appears that the excluded volume effect of the other hemoglobin on Hb S is largely counterbalanced by the solubilizing effect arising from the interaction between the two hemoglobins in solution. The ability of hybrid hemoglobins to gel was demonstrated directly with tetramers in which alpha beta s dimers were covalently linked to alpha beta A, alpha delta A2, and alpha gamma F dimers.
摘要
通过分析离心后上清液和沉淀中S型血红蛋白与非S型血红蛋白的比例，对Hb S与血红蛋白A、A2和F的混合物聚合情况进行了研究。在所有情况下，即使不存在杂种，非S型血红蛋白也会按A > A2 > F的顺序掺入聚合物中。其他血红蛋白，尤其是Hb F，可显著提高Hb S的溶解度，这可以解释其抗镰状细胞作用。似乎其他血红蛋白对Hb S的排阻体积效应在很大程度上被两种血红蛋白在溶液中相互作用产生的增溶效应所抵消。杂种血红蛋白形成凝胶的能力通过四聚体直接得到证明，其中αβs二聚体与αβA、αδA2和αγF二聚体共价连接。
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