Wishe H I, Roy M, Piliero S J
Anat Rec. 1980 Jul;197(3):283-8. doi: 10.1002/ar.1091970302.
Histosol is a non-flammable solvent mixture of synthetic aromatic hydrocarbons with a flash point of 124 degrees F (T.C.C.). It has a lower vapor pressure and evaporation rate than other organic solvents, such as xylene, routinely used as clearing and deparaffinizing agents. Although both xylene and Histosol clear and deparaffinize soft organ tissues effectively in the preparation of permanently mounted stained slides, Histosol appears, in many instances, to be the choice solvent: tissues are easier to section; cell borders and cell surface modifications are most distinct; cytoplasmic eosinophilia is more vivid; and nuclear detail is improved. Of prime importance, Histosol is a safer and more efficient solvent for use in histological and pathological laboratories.
Histosol是一种合成芳烃的不可燃溶剂混合物，闪点为124华氏度（泰格闭口杯法）。它的蒸气压和蒸发速率低于其他通常用作透明剂和脱蜡剂的有机溶剂，如二甲苯。虽然在制备永久封固的染色玻片时，二甲苯和Histosol都能有效地使软组织透明和脱蜡，但在许多情况下，Histosol似乎是首选溶剂：组织更容易切片；细胞边界和细胞表面修饰最为清晰；细胞质嗜酸性更明显；细胞核细节更清晰。最重要的是，Histosol在组织学和病理学实验室中是一种更安全、更高效的溶剂。
