Gilchrest B A, Nemore R E, Maciag T
Cell Biol Int Rep. 1980 Nov;4(11):1009-16. doi: 10.1016/0309-1651(80)90173-3.
Keratinocytes derived from the skin of newborns and of adults aged 19 to 57 years were grown on plates coated with human fibronectin (HFN) in the absence of a 3T3 monolayer. The cells grew well, attained confluence and could be sub-cultivated at densities approximately 10% of those necessary for successful cultivation of human keratinocytes on collagen coated dishes. Growth was excellent at concentrations of fetal bovine serum (FBS) as low as 5%, and appreciable growth occurred over a six day period even in the complete absence of serum. Growth was enhanced by addition of cholera toxin to the medium. Fibroblast overgrowth of the keratinocyte colonies was not observed. The observation that keratinocytes grow well on fibronectin in the absence of a fibroblast feeder-layer should simplify further study of this fastidious cell type and increases our understanding of keratinocyte growth requirements in vitro.
从新生儿以及19至57岁成年人皮肤中获取的角质形成细胞，在涂有人纤连蛋白（HFN）的培养皿上生长，无需3T3单层细胞。细胞生长良好，达到汇合状态，并可在密度约为在胶原包被培养皿上成功培养人角质形成细胞所需密度10%的情况下进行传代培养。在低至5%的胎牛血清（FBS）浓度下生长良好，甚至在完全无血清的情况下，六天内也有明显生长。向培养基中添加霍乱毒素可促进生长。未观察到角质形成细胞集落被成纤维细胞过度生长。角质形成细胞在无成纤维细胞饲养层的情况下能在纤连蛋白上良好生长这一观察结果，应会简化对这种挑剔细胞类型的进一步研究，并增进我们对体外角质形成细胞生长需求的理解。
