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纤连蛋白促进培养的兔角膜上皮在原位迁移。

Fibronectin promotes epithelial migration of cultured rabbit cornea in situ.

作者信息

Nishida T, Nakagawa S, Awata T, Ohashi Y, Watanabe K, Manabe R

出版信息

J Cell Biol. 1983 Nov;97(5 Pt 1):1653-7. doi: 10.1083/jcb.97.5.1653.

DOI:10.1083/jcb.97.5.1653
PMID:6415071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2112701/
Abstract

We investigated the effect of fibronectin on epithelial migration onto the stroma in cultured rabbit cornea. Rabbit plasma fibronectin was purified by affinity chromatography using gelatin-Sepharose 4B, and its purity was confirmed by SDS polyacrylamide slab gel electrophoresis. Antibody against rabbit plasma fibronectin raised in guinea pigs formed a single precipitin line against rabbit plasma and purified rabbit plasma fibronectin by Ouchterlony double diffusion test. When rabbit cornea was cut into small blocks and cultured in TCM-199 medium alone, corneal epithelial cells began to migrate on the cut edge of the corneal stroma. The addition of purified rabbit plasma fibronectin to the culture medium significantly enhanced epithelial migration. The degree of enhancement depended on the amount of fibronectin added. When guinea pig IgG anti-rabbit plasma fibronectin was added, epithelial migration was significantly inhibited when compared with that in control cultured corneal blocks. The results demonstrate that fibronectin promotes epithelial migration in the cornea and thus plays an important role in corneal wound healing.

摘要

我们研究了纤连蛋白对培养的兔角膜上皮向基质迁移的影响。兔血浆纤连蛋白通过使用明胶 - 琼脂糖凝胶4B的亲和层析法进行纯化，其纯度通过SDS聚丙烯酰胺平板凝胶电泳得以确认。在豚鼠体内产生的抗兔血浆纤连蛋白抗体，通过免疫双扩散试验，与兔血浆和纯化的兔血浆纤连蛋白形成单一沉淀线。当将兔角膜切成小块并单独在TCM - 199培养基中培养时，角膜上皮细胞开始在角膜基质的切割边缘迁移。向培养基中添加纯化的兔血浆纤连蛋白可显著增强上皮迁移。增强程度取决于添加的纤连蛋白量。当添加豚鼠抗兔血浆纤连蛋白IgG时，与对照培养的角膜块相比，上皮迁移受到显著抑制。结果表明，纤连蛋白促进角膜上皮迁移，因此在角膜伤口愈合中起重要作用。

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Role of laminin in the attachment of PAM 212 (epithelial) cells to basement membrane collagen.层粘连蛋白在PAM 212（上皮）细胞与基底膜胶原蛋白附着中的作用。
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Fibronectin (LETS) does not support epithelial cell spreading.纤连蛋白（LETS）不支持上皮细胞铺展。
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The cell binding fragment of fibronectin is chemotactic for fibroblasts.纤连蛋白的细胞结合片段对成纤维细胞具有趋化作用。
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Distribution of fibronectin during wound healing in vivo.体内伤口愈合过程中纤连蛋白的分布。
J Invest Dermatol. 1981 Mar;76(3):181-9. doi: 10.1111/1523-1747.ep12525694.
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Rapid preparation of purified autologous fibronectin eyedrops from patient's plasma.从患者血浆中快速制备纯化的自体纤连蛋白眼药水。
Jpn J Ophthalmol. 1982;26(4):416-24.
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Fibronectin and wound healing.纤连蛋白与伤口愈合
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Fibronectin appears at the site of corneal stromal wound in rabbits.纤连蛋白出现在兔角膜基质伤口处。
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