Giuditta A, Rutigliano B, Vitale-Neugebauer A
J Neurochem. 1980 Dec;35(6):1267-72. doi: 10.1111/j.1471-4159.1980.tb08997.x.
Adult male rabbits implanted with dural electrodes were injected intraventricularly with [3H]orotate and killed 1 h later. During the period of incorporation they were left undisturbed while their EEG activity was continuously monitored. In the fraction of neuronal perikarya prepared from cerebral cortex by a method developed by Satake and Abe in 1966, the relative content of radioactive RNA of the nuclear particulate showed a twofold increase in the transition from 0 to 100% synchronization. On the other hand, a slight but significant decline was observed in the corresponding cytoplasmic compartment. A marked increase in the relative content of radioactive RNA was similarly observed in the nuclear particulate prepared from the mixed cellular fraction. The corresponding cytoplasmic compartment showed a nonsignificant increase. These results indicate that during sleep neuronal nuclei accumulate newly synthetised RNA (presumably hnRNA) at a faster rate. Under the same conditions the process of RNA transfer to the cytoplasm (presumably rRNA) may be reduced. These effects may be only partly shared by other cerebral cells.
给植入硬脑膜电极的成年雄性兔子脑室内注射[3H]乳清酸，1小时后处死。在掺入期内，兔子未受干扰，同时持续监测其脑电图活动。在通过坂田和阿部于1966年开发的方法从大脑皮层制备的神经元胞体部分中，核颗粒的放射性RNA相对含量在从0%同步化到100%同步化的转变过程中增加了两倍。另一方面，在相应的细胞质部分观察到轻微但显著的下降。在从混合细胞部分制备的核颗粒中同样观察到放射性RNA相对含量显著增加。相应的细胞质部分显示出不显著的增加。这些结果表明，在睡眠期间，神经元细胞核以更快的速度积累新合成的RNA（可能是hnRNA）。在相同条件下，RNA转移到细胞质（可能是rRNA）的过程可能会减少。其他脑细胞可能只部分具有这些效应。
