Pedersen S M, Graabaek P M
Scand J Clin Lab Invest. 1977 Feb;37(1):91-4.
A new simple non-flame method of assaying serum, urine, and synovial fluid for gold by atomic absorption spectrometry (AAS) is presented. Variable absorption not due to gold is avoided by wet ashing and extraction of gold into methylisobutyl ketone. The amount of gold is determined directly in the organic phase by AAS. A linear relation between standard concentrations for serum, urine, and synovial fluid and absorption meter read-out values is obtained over the ranges 0 to 800 microgram gold/dl in serum and 0 to 80 microgram gold/dl in urine and synovial fluid. The estimated calibration curves for gold in serum, urine, and synovial fluid are found to be identical. The elimination of interferences, the high sensitivity (0.01 ppm), and the simplicity make this method superior to any other AAS methods and excellently applicable for routine determinations of gold concentrations in patients on chrysotherapy, and for research in that field as well.
本文介绍了一种通过原子吸收光谱法（AAS）测定血清、尿液和滑液中金含量的新的简单无火焰方法。通过湿式灰化和将金萃取到甲基异丁基酮中，避免了非金因素引起的可变吸收。金的含量通过AAS直接在有机相中测定。在血清中0至800微克金/分升、尿液和滑液中0至80微克金/分升的范围内，血清、尿液和滑液的标准浓度与吸收计读数之间呈线性关系。发现血清、尿液和滑液中金子的估计校准曲线是相同的。该方法消除了干扰，灵敏度高（0.01 ppm）且操作简单，优于任何其他AAS方法，非常适用于金疗法患者金浓度的常规测定以及该领域的研究。
