Gallyas F
Acta Histochem. 1980;67(1):1-5. doi: 10.1016/S0065-1281(80)80046-8.
Visual end-point "detection" is proposed for the determination of development time. One of adjacent sections is silverized up to an arbitrary intensity and the others treated in various manners for the investigation of certain processes of silver staining are developed until a given area of them equals in intensity, as "detected" by naked eye, with the same area of the control section. Due to the capacity of the human eye "to detect" identity or divergency accurately in light intensity, the error of development time does not exceed the value of 10%. Whereas, alterations of development time caused by the procedure tested mount up even to 300%.
建议采用视觉终点“检测”来确定显影时间。相邻切片中的一张用银盐处理至任意强度，其他切片则采用不同方式处理以研究银染色的某些过程，直至通过肉眼“检测”到它们的给定区域在强度上与对照切片的相同区域相等时显影。由于人眼能够准确“检测”光强度的相同或差异，显影时间的误差不超过10%。然而，所测试程序导致的显影时间变化甚至高达300%。
