福尔根反应：水解后洗涤的重要性
The Feulgen reaction: importance of the wash after hydrolysis.
作者信息
Larson M L, Sauaia H
出版信息
Acta Histochem. 1980;67(1):6-12. doi: 10.1016/S0065-1281(80)80047-X.
PMID:6160716
Abstract
Washing in absolute ethanol after FEULGEN hydrolysis minimizes losses of DNA. The hydrolysis in HCl diluted in tetrahydrofuran is not superior in this respect to conventional hydrolysis if this is followed by an ethanol wash. These conclusions are based on cytophotometric and radiometric experiments made on salivary glands of sciarid larvae.
摘要
福尔根水解后用无水乙醇洗涤可最大程度减少DNA损失。如果随后进行乙醇洗涤，在四氢呋喃中稀释的盐酸水解在这方面并不优于传统水解。这些结论基于对蕈蚊幼虫唾液腺进行的细胞光度法和放射测量实验。
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