[红细胞A抗原的细胞分布异质性。超微结构研究]
[Heterogeneity of the cellular distribution of erythrocytic A antigens. Ultramicroscopic study].
作者信息
Gourdin M F, Reyes F, Lejonc J L, Mannoni P, Breton Gorius J, Dreyfus B
出版信息
Rev Fr Transfus Immunohematol. 1976 Mar;19(1):55-65. doi: 10.1016/s0338-4535(76)80088-8.
A and A1 antigens have been detected on cells of the human erythrocyte series by immunoelectron microscopy. These antigens have been revealed by an indirect method involving various anti-A and anti-A1 antibodies (allo, auto, hetero-antibodies) and peroxidase-conjugated anti-immunoglobulin antibodies. Immunologic labelling has been carried out with erythrocyte or bone marrow cell suspensions which were fixed prior to incubation with reagents. Cells from various A phenotypes were examined. A and A1 antigens were visualized on maturing normoblasts, at every developmental stage. In addition cell to cell variations of the surface labelling of erythrocytes was found in normal phenotypes, suggesting the existence of several populations of cells according to antigenic load.
通过免疫电子显微镜已在人类红细胞系列细胞上检测到A和A1抗原。这些抗原通过一种间接方法得以揭示，该方法涉及各种抗A和抗A1抗体（同种、自身、异种抗体）以及过氧化物酶偶联的抗免疫球蛋白抗体。免疫标记是在与试剂孵育之前固定的红细胞或骨髓细胞悬液上进行的。检查了来自各种A血型的细胞。在每个发育阶段的成熟成红细胞上都可看到A和A1抗原。此外，在正常血型中发现红细胞表面标记存在细胞间差异，这表明根据抗原负荷存在几种细胞群体。
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