Ward R, Kohler H
J Immunol. 1981 Jan;126(1):146-9.
The existence of precursors for TI and TD alpha 1 leads to 3 dextran antigens in BALB/c mice was demonstrated. A T-dependent dextran antigen was prepared by coupling dextran B1355S to hemocyanin and subsequent digestion with dextranase. The PFC response of BALB/c mice primed with hemocyanin to dextran-hemocyanin was found to be 8 times higher than in unprimed animals. The splenic focus assay was adapted for the analysis of precursors responding to T-dependent and T-independent dextran antigens. Pretreatment of recipients with anti-thymocyte serum abolished the response in fragment cultures to dextran-hemocyanin but did not affect the response to dextran B1355S. The frequencies of precursors in the adult BALB/c mouse responding to dextran and dextran-hemocyanin were determined by limiting dilution analysis. The frequency of T-dependent precursors was found to be almost 3 times greater than the frequency of T-independent precursors.
已证明在BALB/c小鼠中存在TI和TDα1的前体，可产生3种葡聚糖抗原。通过将葡聚糖B1355S与血蓝蛋白偶联，随后用葡聚糖酶消化，制备了一种T依赖性葡聚糖抗原。发现用葡聚糖-血蓝蛋白致敏的血蓝蛋白的BALB/c小鼠的PFC反应比未致敏动物高8倍。脾灶试验适用于分析对T依赖性和T非依赖性葡聚糖抗原作出反应的前体。用抗胸腺细胞血清对受体进行预处理可消除片段培养物中对葡聚糖-血蓝蛋白的反应，但不影响对葡聚糖B1355S的反应。通过有限稀释分析确定成年BALB/c小鼠中对葡聚糖和葡聚糖-血蓝蛋白作出反应的前体频率。发现T依赖性前体的频率几乎比T非依赖性前体的频率大3倍。
