Morello D, Condamine H, Delarbre C, Babinet C, Gachelin G
J Exp Med. 1980 Dec 1;152(6):1497-505. doi: 10.1084/jem.152.6.1497.
Using an affinity chromatography technique, IgM, IgG1, IgG2a,b anti-F9 antibodies have been isolated from the anti-F9 serum; their activities have been analyzed by IF test on a variety of cell types, teratocarcinoma-derived cell lines, and embryos. The anti-F9 antibodies react with at least three independent antigenic determinants not expressed on the same cell types, and that appear along different time-course during embryonic development.
利用亲和层析技术,已从抗F9血清中分离出IgM、IgG1、IgG2a和IgG2b抗F9抗体;通过免疫荧光试验在多种细胞类型、畸胎瘤衍生细胞系和胚胎上分析了它们的活性。抗F9抗体与至少三种独立的抗原决定簇发生反应,这些抗原决定簇在不同细胞类型上不表达,且在胚胎发育过程中呈现出不同的时间进程。