青蛙和大鼠感觉神经元中快速运输轴突蛋白的比较分析。

Comparative analysis of rapidly transported axonal proteins in sensory neurons of the frog and rat.

作者信息

Neale J H, Forman D S, Shibla D B, Shortell S A

出版信息

J Neurochem. 1980 Oct;35(4):838-42. doi: 10.1111/j.1471-4159.1980.tb07080.x.

DOI:10.1111/j.1471-4159.1980.tb07080.x

Abstract

35S-labeled proteins carried by fast axonal transport in sciatic sensory axons of bullfrog and rat were separated electrophoretically on discontinuous polyacrylamide gradient slab gels. In contrast to the previously reported similarity in the electrophoretic profiles of rapidly transported proteins from functionally different neurons, we have found that there is very little correspondence in the profiles of these proteins in functionally similar neurons from two widely studied species. We also found very little correspondence between the two species in the profiles of locally synthesized sciatic nerve protein. The results demonstrate the difficulty inherent in comparing the electrophoretic profiles obtained using these two model systems for the study of rapidly transported axonal proteins. In particular, relationships between the major rapidly transported proteins in the two species could not be analyzed with this technique.

摘要

对牛蛙和大鼠坐骨神经感觉轴突中通过快速轴突运输携带的35S标记蛋白质，在不连续聚丙烯酰胺梯度平板凝胶上进行电泳分离。与之前报道的功能不同神经元中快速运输蛋白质的电泳图谱相似性相反，我们发现来自两个广泛研究物种的功能相似神经元中，这些蛋白质的图谱几乎没有对应关系。我们还发现，在局部合成的坐骨神经蛋白质图谱方面，这两个物种之间也几乎没有对应关系。结果表明，使用这两种模型系统研究快速运输的轴突蛋白时，比较所得电泳图谱存在内在困难。特别是，无法用该技术分析这两个物种中主要快速运输蛋白质之间的关系。