Kamata S, Okada A, Watanabe T, Kawashima Y, Wada H
J Neurochem. 1980 Nov;35(5):1190-9. doi: 10.1111/j.1471-4159.1980.tb07875.x.
The etiologic relationship between disturbances in metabolism of amino acids and amines and hepatic coma was investigated by examining the effects of diets containing various mixtures of amino acids on brain amine metabolism in rats with a portacaval shunt, using a method for simultaneous analysis of amino acids and amines. Rats with a portacaval shunt were fed on four different amino acid compositions with increased amounts of various amino acids suspected to be etiologically related to hepatic coma, such as methionine, phenylalanine, tyrosine, and tryptophan. The animals were killed 4 weeks after operation. During the experimental period, these animals did not become comatose, but exhibited various behavioral abnormalities. Marked increase in the plasma and brain levels of the augmented amino acids, especially methionine and tyrosine, were observed in rats with a portacaval shunt. Brain noradrenaline, dopamine, and serotonin levels were significantly decreased when the brain tyrosine level was increased. These results indicate that in rats with a portacaval shunt the dietary levels of amino acids greatly influence the brain levels of both amino acids and transmitter amines.
通过使用一种同时分析氨基酸和胺类的方法，研究含有各种氨基酸混合物的饮食对门腔分流大鼠脑胺代谢的影响，探讨氨基酸和胺类代谢紊乱与肝昏迷之间的病因关系。给门腔分流大鼠喂食四种不同氨基酸组成的食物，其中怀疑与肝昏迷病因相关的各种氨基酸含量增加，如蛋氨酸、苯丙氨酸、酪氨酸和色氨酸。术后4周处死动物。在实验期间，这些动物未出现昏迷，但表现出各种行为异常。在门腔分流大鼠中，观察到血浆和脑中增加的氨基酸水平显著升高，尤其是蛋氨酸和酪氨酸。当脑酪氨酸水平升高时，脑去甲肾上腺素、多巴胺和5-羟色胺水平显著降低。这些结果表明，在门腔分流大鼠中，饮食中的氨基酸水平极大地影响脑中氨基酸和递质胺类的水平。
