Chu A C, Smith N, MacDonald D M
Br J Dermatol. 1980 Dec;103(6):607-13. doi: 10.1111/j.1365-2133.1980.tb01682.x.
Biopsies from lesional skin of patients with histologically confirmed mycosis fungoides were embedded in resin and semi-thin ( I micrometer) sections were submitted to a variety of staining procedures, including Toluidine blue, Richardson's Huber, Giemsa, Paragon and Tribasic stains. The stained sections were examined with the light microscope and the quality of cellular morphological detail afforded by the different stains was assessed. The Toluidine blue, Richardson's and Paragon stains were found to be technically easy to perform and gave the best representation of nuclear and cytoplasmic detail Of these, the authors favoured Richardson's stain. The technique of processing the tissue, and staining a mounting procedures are detailed.
对组织学确诊为蕈样肉芽肿患者的皮损活检标本进行树脂包埋，制备半薄（1微米）切片，并进行多种染色程序，包括甲苯胺蓝染色、理查森氏休伯染色、吉姆萨染色、帕拉贡染色和三元染色。对染色后的切片进行光学显微镜检查，并评估不同染色所提供的细胞形态细节质量。结果发现，甲苯胺蓝染色、理查森氏染色和帕拉贡染色在技术上易于操作，并且能最好地呈现细胞核和细胞质细节。在这些染色方法中，作者更倾向于理查森氏染色。本文详细介绍了组织处理技术以及染色和封片程序。
