Lachmann P J, Oldroyd R G, Milstein C, Wright B W
Immunology. 1980 Nov;41(3):503-15.
Three monoclonal antibodies to human C3 have been obtained from a fusion of the rat myeloma line Y3 Ag 1.2.3. with spleen cells from rats immunized against C3. One, from clone 4, reacts with an antigenic determinant in C3c showing the expected reactivity of the 'C' antigen of C3. The specificity of the other two monoclonal antibodies correspond less clearly with known C3 antigens. By agglutination analysis of complement coated cells the determinant reacting with clone 3 is present in C3d while that for clone 9 appears as a neoantigen on C3bi. In both cases the co-precipitation results are anomalous and more direct studies are needed to define the exact specificity. The possibility that internal sequence duplications in C3 may explain some anomalies is discussed. None of the monoclonal antibodies significantly inhibit C3 functions. The monoclonal antibodies have been found to have unusual properties in co-precipitation assays being able to diffuse through a precipitation line with which they react to react with a further line. One antibody is also able to react strongly with the anodal half of what appears as a single line with a polyclonal antiserum.
通过将大鼠骨髓瘤细胞系Y3 Ag 1.2.3. 与用C3免疫的大鼠脾细胞进行融合，已获得三种针对人C3的单克隆抗体。其中一种来自克隆4，与C3c中的一个抗原决定簇发生反应，表现出C3的“C”抗原预期的反应性。另外两种单克隆抗体的特异性与已知的C3抗原不太相符。通过对补体包被细胞的凝集分析，与克隆3反应的决定簇存在于C3d中，而克隆9的决定簇则表现为C3bi上的一种新抗原。在这两种情况下，共沉淀结果都不正常，需要更直接的研究来确定确切的特异性。讨论了C3内部序列重复可能解释一些异常现象的可能性。这些单克隆抗体均未显著抑制C3功能。已发现这些单克隆抗体在共沉淀试验中具有不寻常的特性，能够扩散穿过与其反应的沉淀线，与另一条线发生反应。一种抗体还能与用多克隆抗血清呈现为单一条带的阳极半部分强烈反应。
