Lansdorp P M, Astaldi G C, Oosterhof F, Janssen M C, Zeijlemaker W P
J Immunol Methods. 1980;39(4):393-405. doi: 10.1016/0022-1759(80)90240-9.
An immunoperoxidase method has been developed which allows accurate and sensitive quantitation of the binding of monoclonal antibodies to cell surface antigens. Monolayers of fixed cells were prepared in wells of Terasaki micro-test plates and monoclonal antibodies bound to cell surface antigens were identified by the unlabeled antibody-enzyme method of Sternberger (1974). The cell-bound peroxidase could either be quantified per well or visualized on individual cells by the use of appropriate substrates for peroxidase. Experimental procedures are described in detail and results obtained with several monoclonal antibodies with specificity for different target cells are shown. Limitations and applications of the technique are discussed.
已开发出一种免疫过氧化物酶方法，该方法可对单克隆抗体与细胞表面抗原的结合进行准确且灵敏的定量分析。在特拉斯基微量测试板的孔中制备固定细胞单层，采用斯特恩伯格（1974年）的未标记抗体-酶法鉴定与细胞表面抗原结合的单克隆抗体。细胞结合的过氧化物酶既可以按孔进行定量分析，也可以通过使用过氧化物酶的合适底物在单个细胞上进行可视化观察。详细描述了实验步骤，并展示了用几种对不同靶细胞具有特异性的单克隆抗体所获得的结果。讨论了该技术的局限性和应用。
