Satz M L, Sztein M B, Serrate S, Braun M
Mol Cell Biochem. 1980 Dec 16;33(3):105-13. doi: 10.1007/BF00225282.
Immune RNA (I-RNA) was extracted form lymphoid organs of BALB/c mice immunized with AKR lymphoid cells. Incubation of normal BALB/c spleen cells with this I-RNA (but not with normal RNA) resulted in leukocyte migration inhibition reactions (LMIR) against AKR extracts but not against purified protein derivative or BALB/c sarcoma extracts. This transfer was abolished by pretreating I-RNA with RNAse but not with pronase. The active fraction of I-RNA was retained by and could be eluted from Poly-U Sepharose columns. Normal cells pretreated with I-RNA also reacted in the presence of an anti-idiotypic anti-serum of anti-(BALB/c anti-AKR) specificity. Pretreatment of cells with anti-idiotypic serum plus complement did not inhibit the subsequent transfer of LMIR with I-RNA. Idiotypic receptors were expressed on I-RNA treated cells less than one hour after I-RNA treatment. Using an I-RNA of double specificity, the results suggested that I-RNA entered into and acted on the cells through a nonspecific mechanism. Finally, I-RNA could induce BALB/c anti-AKR idiotypic markers in C57Bl/6 cells, genetically committed for different idiotypes, while RNA extracted from C57Bl/6 immune cells could not induce in BALB/c cells their own genetically acquired idiotypes. This series of data would prove that I-RNA acting as a mRNA is able to induce in normal noncommitted cells the de novo synthesis of antigen receptors similar or identical to those present in the surface of in vivo immunized lymphoid cells of the same strain.
免疫核糖核酸（I-RNA）是从用AKR淋巴细胞免疫的BALB/c小鼠的淋巴器官中提取的。用这种I-RNA（而非正常RNA）孵育正常BALB/c脾细胞，会导致针对AKR提取物的白细胞迁移抑制反应（LMIR），但对纯化蛋白衍生物或BALB/c肉瘤提取物则无此反应。用核糖核酸酶预处理I-RNA可消除这种转移作用，而用蛋白酶处理则无此效果。I-RNA的活性部分可被聚尿苷琼脂糖柱保留并能从该柱上洗脱下来。用I-RNA预处理的正常细胞在存在具有抗-（BALB/c抗-AKR）特异性的抗独特型抗血清时也会发生反应。用抗独特型血清加补体预处理细胞并不会抑制随后用I-RNA进行的LMIR转移。在I-RNA处理后不到一小时，独特型受体就在经I-RNA处理的细胞上表达。使用具有双重特异性的I-RNA，结果表明I-RNA通过非特异性机制进入细胞并在细胞上发挥作用。最后，I-RNA可在基因上决定具有不同独特型的C57Bl/6细胞中诱导出BALB/c抗-AKR独特型标记，而从C57Bl/6免疫细胞中提取的RNA则不能在BALB/c细胞中诱导出其自身基因获得的独特型。这一系列数据将证明，作为信使核糖核酸发挥作用的I-RNA能够在正常未定向细胞中诱导从头合成与同一品系体内免疫的淋巴细胞表面存在的抗原受体相似或相同的抗原受体。
