[小鼠干扰素信使核糖核酸在无细胞系统中的翻译]
[Translation of mouse interferon messenger RNA in a cell-free system].
作者信息
Kisling U, Sokolova T M, Ershov F I
出版信息
Biull Eksp Biol Med. 1980 Dec;90(12):675-7.
PMID:6162490
Abstract
Crude mouse interferon mRNA extracted from poly (I) . poly (C)-induced L929 cells has been translated in a cell-free system from rabbit reticulocytes and in two-cell systems--L929 cells and chick embryo fibroblasts. Translational efficiency of interferon mRNA preparation was 100-fold higher in the cell-free system than in tissue culture cells. Interferon synthesized was species-specific and its antiviral activity was completely neutralized by mouse interferon antiserum.
摘要
从聚（I）·聚（C）诱导的L929细胞中提取的粗制小鼠干扰素mRNA已在兔网织红细胞的无细胞系统以及双细胞系统（L929细胞和鸡胚成纤维细胞）中进行了翻译。干扰素mRNA制剂在无细胞系统中的翻译效率比在组织培养细胞中高100倍。合成的干扰素具有种属特异性，其抗病毒活性被小鼠干扰素抗血清完全中和。
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[Translation of mouse interferon messenger RNA in a cell-free system].[小鼠干扰素信使核糖核酸在无细胞系统中的翻译]
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