Litwin J A, Kasprzyk J M
Folia Histochem Cytochem (Krakow). 1980;18(4):245-8.
Hale's colloidal iron reaction for acid mucopolysaccharides was applied to semithin sections of Epon-embedded tissues routinely processed for electron microscopy. The reaction was found to be positive only after partial removal of the resin in NaOH/absolute ethanol solution; its sensitivity, however, did not reach that obtained in paraffin or frozen sections. The duration of the Epon-removing procedure seems to be crucial for the specificity of staining.
将黑尔用于酸性黏多糖的胶体铁反应应用于常规处理用于电子显微镜检查的环氧树脂包埋组织的半薄切片。发现该反应仅在NaOH/无水乙醇溶液中部分去除树脂后呈阳性；然而，其灵敏度未达到石蜡切片或冷冻切片的灵敏度。去除环氧树脂的程序持续时间似乎对染色特异性至关重要。
