Kawanishi K, Nishina Y, Ishida T, Machida S, Yamamoto S, Ofuji T, Sakura N, Yanaihara N
Horm Metab Res. 1980 Dec;12(12):660-4. doi: 10.1055/s-2007-999227.
C-peptide immunoreactivity (CPR) levels were measured in dog superior pancreaticoduodenal vein using synthetic dog C-peptide and its antiserum. The basal CPR level was approximately twice as high as the basal immunoreactive insulin (IRI) level on a molar basis. Glucose (10 mg/kg/min) or arginine (250 mg/kg/min) infusion for 5 min into the superior pancreaticoduodenal artery caused a prompt, parallel increase in IRI and CPR. IRI and CPR were closely equimolar at peak secretions. One bolus administration of synthetic neurotensin (10 microgram/kg) into the same artery produced a mild hyperglycemic response and biphasic IRI and CPR responses at 30 min in the vein. The IRI and CPR increases were closely equimolar during the first phase of secretion, but during the second peak a larger increase was found in CPR than IRI. Upon infusion of synthetic substance P (50 ng/kg/min) for 30 min, IRI and CPR concentrations showed a parallel and closely equimolar fall. These results indicate that insulin and C-peptide were released from beta cells in equimolar concentrations.
使用合成犬C肽及其抗血清，测量犬胰十二指肠上静脉中的C肽免疫反应性（CPR）水平。在摩尔基础上，基础CPR水平约为基础免疫反应性胰岛素（IRI）水平的两倍。向胰十二指肠上动脉内输注葡萄糖（10mg/kg/min）或精氨酸（250mg/kg/min）5分钟，可使IRI和CPR迅速、平行升高。在分泌峰值时，IRI和CPR的摩尔量相近。向同一动脉内一次性注射合成神经降压素（10μg/kg），30分钟时在静脉中产生轻度高血糖反应以及双相IRI和CPR反应。在分泌的第一阶段，IRI和CPR的升高摩尔量相近，但在第二个峰值时，CPR的升高幅度大于IRI。输注合成P物质（50ng/kg/min）30分钟后，IRI和CPR浓度呈平行且相近摩尔量下降。这些结果表明，胰岛素和C肽以等摩尔浓度从β细胞释放。
