Dräger U C, Olsen J F
Invest Ophthalmol Vis Sci. 1981 Mar;20(3):285-93.
The distribution of ganglion cells in the mouse retina was studied with the use of Nissl criteria for distinguishing cell types in the ganglion cell layer. Retrograde filling with horseradish peroxidase (HRP) from the optic fiber tract helped to validate Nissl criteria and served to identify displaced ganglion cells. We estimated a total of 117,000 nonvascular cells in the ganglion cell layer; of these, 70,000 were probably ganglion cells, and 47,000 could not be classified. The density of the presumed ganglion cells was highest-more than 8000 cells/mm2-just temporal to the optic disk, and lowest-less than 2000 cells/mm2-in the most dorsal retina. The retinal region with highest ganglion cell density was slightly elongated in a nasotemporal direction. About 2% of all HRP-filled ganglion cells had their cell bodies in the inner nuclear layer. These displaced cells differed in topographical distribution from the normally positioned ganglion cells: although occurring throughout the retina, they were more common along the retinal periphery. Measurements of ganglion cell areas showed a tendency toward larger size with eccentricity. At no retinal location did cell-size histograms reveal clearly separate size classes.
利用尼氏染色法区分神经节细胞层中的细胞类型，研究了小鼠视网膜中神经节细胞的分布情况。从视神经纤维束进行辣根过氧化物酶（HRP）逆行填充，有助于验证尼氏染色法，并用于识别移位的神经节细胞。我们估计神经节细胞层共有117,000个非血管细胞；其中，70,000个可能是神经节细胞，47,000个无法分类。推测的神经节细胞密度在紧邻视盘颞侧处最高，超过8000个细胞/mm²，而在视网膜最靠背侧处最低，低于2000个细胞/mm²。神经节细胞密度最高的视网膜区域在鼻颞方向略呈细长形。所有HRP填充的神经节细胞中约2%的细胞体位于内核层。这些移位细胞的地形分布与正常定位的神经节细胞不同：尽管在整个视网膜中都有出现，但在视网膜周边更为常见。神经节细胞面积的测量显示，随着离心率增加，细胞有变大的趋势。在视网膜的任何位置，细胞大小直方图都未显示出明显分开的大小类别。
