Babb J L, Cummings C S
J Clin Microbiol. 1981 Feb;13(2):369-79. doi: 10.1128/jcm.13.2.369-379.1981.
The serological properties of antigens extracted from strains of Bacteroides fragilis and related species belonging to several different deoxyribonucleic acid homology groups were investigated. Antisera prepared against Formalin-treated whole cell suspensions of representative strains were tested against cell suspensions, cell wall preparations, and extracts of homologous and heterologous strains by using agglutination, immunodiffusion, and hemagglutination techniques. Serological results indicated that the species were antigenically distinct, although minor cross-reactions were observed. Homology groups, including the two B. fragilis subgroups, were relatively homogeneous, although the presence of serotypes within each homology group was suggested. Immunodiffusion tests demonstrated, however, that each possessed a mosaic antigen composition; at least 6 antigenic determinants could be demonstrated in B. fragilis 2553, and up to 10 were found in B. fragilis 2393. Hemagglutination tests using antigen extracts also indicate a mosaic of antigens in each strain.
对从脆弱拟杆菌菌株及属于几个不同脱氧核糖核酸同源组的相关物种中提取的抗原的血清学特性进行了研究。用针对代表性菌株的福尔马林处理全细胞悬液制备的抗血清，通过凝集、免疫扩散和血凝技术，对同源和异源菌株的细胞悬液、细胞壁制剂及提取物进行检测。血清学结果表明，尽管观察到轻微的交叉反应，但这些物种在抗原性上是不同的。包括两个脆弱拟杆菌亚组在内的同源组相对均一，不过提示每个同源组内存在血清型。然而，免疫扩散试验表明，每个都具有镶嵌抗原组成；在脆弱拟杆菌2553中可证明至少有6个抗原决定簇，在脆弱拟杆菌2393中发现多达10个。使用抗原提取物的血凝试验也表明每个菌株中存在抗原镶嵌现象。
