Itoh M, Nakashima T
Acta Otolaryngol. 1980 Nov-Dec;90(5-6):385-90. doi: 10.3109/00016488009131739.
Rootlets of the sensory hairs of the basal turn of the guinea pig cochlea were investigated using the tannic acid fixation method under transmission electron microscopy (TEM). The fine structure of the rootlet was enhanced by adding tannic acid to the prefixative for the specimen. By using the tannic acid fixation method, it is possible to observe the finer filamentous structure of the rootlet in greater detail than is revealed by using the ordinary glutaraldehyde-osmium fixation method. TEM observation by the tannic acid method reveals that the rootlet consists of 50 A diameter protofilaments and an electron-dense background substance. These protofilaments are arranged in an extremely regular pattern. They are considered to be modified microfilaments.
采用单宁酸固定法，在透射电子显微镜（TEM）下对豚鼠耳蜗底转感觉毛的根丝进行了研究。通过在标本的预固定剂中添加单宁酸，增强了根丝的精细结构。使用单宁酸固定法，可以比普通的戊二醛-锇固定法更详细地观察根丝更精细的丝状结构。单宁酸法的TEM观察表明，根丝由直径50埃的原丝和电子致密的背景物质组成。这些原丝以极其规则的模式排列。它们被认为是修饰后的微丝。
