Chaplin A J
Stain Technol. 1985 Jul;60(4):219-31. doi: 10.3109/10520298509113916.
The development of tannic acid as a reagent in histological methods is traced against a background of widespread use in science and technology from times of antiquity. Numerous light microscopic methods involving tannic acid, particularly in conjunction with iron and silver, have been described for a variety of tissue components. In most applications, tannic acid functions as a mordant. Current use is generally restricted to methods based on its affinity for collagen. The most significant histological use of tannic acid in contemporary times is as an adjunct to conventional glutaraldehyde-osmium-heavy metal fixation and staining for ultrastructural studies of tissue structures not normally clearly demonstrated. Tannic acid reacts with various components by mechanisms which are often not fully understood.
单宁酸作为一种组织学方法中的试剂，其发展历程是以自古以来在科学技术中的广泛应用为背景的。已经描述了许多涉及单宁酸的光学显微镜方法，特别是与铁和银结合使用时，用于各种组织成分。在大多数应用中，单宁酸起媒染剂的作用。目前其用途一般限于基于其对胶原蛋白亲和力的方法。当代单宁酸在组织学上最重要的用途是作为传统戊二醛 - 锇 - 重金属固定和染色的辅助剂，用于对通常无法清晰显示的组织结构进行超微结构研究。单宁酸通过一些通常尚未完全理解的机制与各种成分发生反应。
