Stimulation by calcium chloride of virus-induced interferon formation by blood, haemopoietic organ and continuous human cells.

Calcium chloride stimulated virus-induced production of leukocyte interferon by human and animal blood and haemopoietic organ cells. CaCl2 treatment of surviving cells (leukocytes, human and mouse bone marrow) and Namalva cells was the most effective when carried out simultaneously with adsorption of the virus-inducer or when CaCl2 pretreatment was combines with its subsequent addition together with the virus-inducer. Optimal CaCl2 concentrations were 5 mM for human bone marrow cells and 10 mM for human leukocytes and mouse bone marrow cells. CaCl2 treatment was equivalent to priming in case of interferon induction in human leukocytes and, as distinct from priming, it considerably increased virus-induced interferon production by Namalva cells.