Mallet J, Privat A, Samolyk D, Bérod A, Keller A, Pujol J F
C R Seances Acad Sci III. 1981 Feb 9;292(6):455-60.
RNA from PC 12 cells was translated in a cell-free system and the peptides synthesized in vitro were analyzed by immunoprecipitation with an anti-T.H. serum raised in Rabbit. Analysis of the immunoprecipitate on a SDS-polyacrylamide gel reveals a major band with a molecular weight of 62000 dalton. A protein of the same molecular weight was also precipitated from a PC12 cell lysate. This molecular weight of 62000 dalton corresponds to the reported for tyrosine hydroxylase purified from PC12 cells.
来自PC12细胞的RNA在无细胞系统中进行翻译，体外合成的肽段用兔抗T.H.血清通过免疫沉淀法进行分析。在SDS-聚丙烯酰胺凝胶上对免疫沉淀物进行分析，显示出一条分子量为62000道尔顿的主要条带。同样分子量的一种蛋白质也从PC12细胞裂解物中沉淀出来。62000道尔顿的这个分子量与从PC12细胞中纯化的酪氨酸羟化酶所报道的分子量相对应。
