金精三羧酸对卤虫无节幼体分离细胞核中依赖DNA的RNA聚合酶II活性的优先刺激作用。

Preferential stimulation by aurintricarboxylic acid of DNA-dependent RNA polymerase II activity in isolated larval nuclei of Artemia salina.

作者信息

Swennen L, Moens L, Heip J, Kondo M

出版信息

Hoppe Seylers Z Physiol Chem. 1981 Feb;362(2):133-42. doi: 10.1515/bchm2.1981.362.1.133.

DOI:10.1515/bchm2.1981.362.1.133

Abstract

Aurintricarboxylic acid was found to stimulate preferentially RNA polymerase II activity in isolated nuclei of Artemia salina larvae. Studies on the in vitro transcription of a homologous chromatin indicate that this dye induces changes in the chromatin so as to enhance its template capacity for free RNA polymerase. Both free RNA polymerases I and II are equally sensitive to this dye in the absence of chromatin template. RNA polymerase II, however, becomes highly resistant to aurintricarboxylic acid when it is actively transcribing the chromatin template, whereas RNA polymerase I remains extremely sensitive even in the transcription complex. Therefore, in isolated nuclei, a preferential stimulation of alpha-amanitin-sensitive, but not-resistant RNA synthesis by aurintricarboxylic acid can be observed.

摘要

发现金精三羧酸能优先刺激卤虫幼虫分离细胞核中的RNA聚合酶II活性。对同源染色质体外转录的研究表明，这种染料会诱导染色质发生变化，从而增强其对游离RNA聚合酶的模板能力。在没有染色质模板的情况下，游离的RNA聚合酶I和II对这种染料同样敏感。然而，当RNA聚合酶II积极转录染色质模板时，它对金精三羧酸变得高度耐药，而RNA聚合酶I即使在转录复合物中仍极其敏感。因此，在分离的细胞核中，可以观察到金精三羧酸对α-鹅膏蕈碱敏感而非耐药的RNA合成有优先刺激作用。